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ACCE
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Chemical ecology platform
GC-MS
The bioactive compounds identified through GC-EAD are further analyzed using gas chromatography-mass spectrometry (GC-MS), which provides precise identification of the chemical structure and molecular weight of these compounds. This allows for the confirmation of the chemical identity of the attractants and quantification of their concentrations.
GC-EAD
Gas-chromatograph electroantennographic detection (GC-EAD) is used to determine which of the sampled compounds elicit a response from insect antennae. The antennae of the target insect species are exposed to the output of the GC, and their electrical responses are recorded, identifying bioactive compounds such as potential attractants.
Sampling facility
Behavioural room
A sampling facility collects volatile organic compounds (VOCs) from plants, insects, or the surrounding environment, typically through techniques like headspace sampling, solid-phase microextraction (SPME), or air entrainment. Once collected, these VOCs are introduced into a gas chromatograph (GC) to separate the complex mixture into individual components.
Laboratory behavioral tests, such as Y-tube olfactometers and wind tunnels, play a critical role in evaluating the efficacy of natural attractants identified through GC-EAD and GC-MS. Wind tunnel or Y-tube olfactometer tests for example are essential for confirming that the identified attractants not only elicit a physiological response but also trigger the desired behavioral outcomes, such as attraction, orientation, or landing.
12 avril 2025
Biocontrol facility
Insect rearing faciliy
Quarantine facility
Artificial Diet room
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